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The communication of the Keithley 2000 Digital Multimeter and PC, and the automatic batch acquisition of the 10 channel 4 wire soil temperature sensors are realized, based on the serial communication subroutine of VB, by means of the model multiplexing scanner card of the Keithley 2000 It solved the existing problems of channel errors and low precision in the calibration of platinum resistance temperature sensors using automatic weather station data loggers in laboratory. The practical application shows that this system can provide high accuracy test results in calibrating soil temperature sensors, fast real time data refreshing, and improved working efficiency.